This laboratory course is designed to provide extensive hands on application of standard protein biotechnology laboratory techniques. This is the second of two sequential Biotechnology laboratory courses. The primary focus of this course is basic protein biochemistry concepts and techniques. Practical, hands-on instruction will provide students with basic skills preparing them for job placement as an entry level laboratory technician. These skills include but are not limited to following and developing standard operating protocols, keeping accurate lab and management records, following state/national laboratory safety procedures and implementing laboratory-relevant mathematics used in routine chemical preparation, experimental procedures and data analysis. Laboratory techniques will focus on protein structure, isolation, purification and characterization. Experimentation areas will focus on immunobiological assays, including the use of monoclonal and polyclonal antibodies in western blotting and antigen detection assays. Protein separation, analysis and interactions will also be addressed. Proficiency in use of common biotechnology lab equipment and data analysis instrumentation will also be emphasized.
Prerequisite: BSC 2420 with a minimum grade of C and BSC 2426C with a minimum grade of C and (BSC 2010 and BSC 2010L or BSC 2010CH with a minimum grade of C)